MPSC Assistant Chemical Analyser Syllabus 2026 Download Group B Exam Pattern
Maharashtra Public Service Commission (MPSC) has released the syllabus for the post of Assistant Chemical Analyser under the Directorate of Forensic Science Laboratories (DFSL), Home Department, Government of Maharashtra. The post belongs to General State Services, Group-B. The MPSC Assistant Chemical Analyser Syllabus 2026 covers four major areas: Analytical Chemistry, Inorganic Chemistry Techniques, Organic Chemistry Techniques, and Biochemistry Techniques. Candidates preparing for the examination should carefully study all the topics given in the official syllabus.
MPSC Assistant Chemical Analyser Exam Overview 2026
| Post Name | Assistant Chemical Analyser |
| Department | Directorate of Forensic Science Laboratories (DFSL), Home Department, Government of Maharashtra |
| Service Category | General State Services, Group-B |
| Number of Subjects/Units | 4 |
| Subjects / Units | Analytical Chemistry, Inorganic Chemistry Techniques, Organic Chemistry Techniques, Biochemistry Techniques |
| Total Questions | 100 |
| Total Marks | 200 |
| Exam Duration | 1 Hour |
MPSC Assistant Chemical Analyser Syllabus 2026: Subject-Wise Syllabus
The MPSC Assistant Chemical Analyser syllabus is divided into four major units covering analytical chemistry fundamentals and their applications in inorganic, organic and biochemical sample analysis.
1. Analytical Chemistry
(A) Basic Aspects
- Sampling of solid, liquid, and gas samples
- Molecular and equivalent weight
- Molarity, molality, normality
- Solution preparation in w/w, w/v, and v/v terms
- Mole concept, concentrations, ppm, ppb, and ppt
- Calibration and validation: calibration curve, regression analysis, external standard, internal standard, and standard addition method
(B) Statistical Treatment of Results
- Accuracy, precision, sensitivity, detection limit
- Errors and their types, mean, median, standard deviation, relative standard deviation
- Gaussian curve, t-test, F-test, confidence interval
- Criteria for selection of analytical methods: accuracy, precision, sensitivity, selectivity, specificity, detection limit
(C) Titrimetry
| Types of titrations | acid-base, complexometric, redox, precipitation |
|---|---|
| Standards used for standardisation | primary standard and secondary standard |
| Potentiometric and conductometric titration and their applications | |
(D) Gravimetric and Thermogravimetric Analysis
- Precipitation and different precipitants used in gravimetric analysis
- pH requirement for precipitation
- Quantitative analysis
- Thermogravimetric analysis
- Mass spectrometry for qualitative and quantitative analysis, and related problems
(E) Separation Techniques
- Extraction methods
- Distillation methods: vacuum distillation, steam distillation, fractional distillation
- Purification and identification of substances
2. Analytical Techniques in Inorganic Chemistry for Different Inorganic Samples
(A) Spectroscopy and Chromatography for Inorganic Sample Analysis
- Atomic absorption, atomic emission, and inductively coupled plasma-atomic emission and mass spectrometry (ICP-AES and ICP-MS)
- UV-Visible spectroscopy for qualitative and quantitative analysis, Job’s Method, and applications
- Ion exchange chromatography for analysis of various inorganic cations and anions
(B) Microscopy for Inorganic Sample Analysis
- Trace and heavy metal, glass, soil, and liquid sample analysis using Scanning Electron Microscopy (SEM)
- Energy Dispersive Spectroscopy (EDS)
(C) Electroanalytical Techniques
- Coulometry and its types
- Amperometry, voltammetry, cyclic voltammetry, stripping voltammetry
- Polarography
3. Analytical Techniques in Organic Chemistry
(A) Spectroscopy
- Theory and application of UV-Visible, IR (FTIR), Raman, NMR, and Mass spectrometry
- Interpretation of spectra and structure elucidation
(B) Separation Techniques
- Theory and application in chemical analysis of TLC, HPTLC, GC, GC-MS, LC-MS, HRMS, column chromatography, and paper chromatography
- HPLC: ion exchange chromatography, adsorption chromatography, normal and reverse phase chromatography, solid phase extraction
(C) Organic Analysis
- Pharmaceutical and narcotic drugs, psychotropic substances
- Alcohol estimation
- Petroleum products, paints and dyes, rubber, plastic, fibres
- Organic poisons such as insecticides, pesticides, herbicides, etc.
4. Analytical Techniques in Biochemistry
(A) Biomolecules
- Structure, functions, and estimation of carbohydrates, lipids, proteins, nucleic acids
- Biological buffers
(B) Molecular Biology
- DNA isolation, DNA quantification
- PCR, RT-PCR, RFLP (Restriction Fragment Length Polymorphism)
- ELISA, RIA (Radio Immuno Assay)
- Antigen-antibody reactions
(C) Separation Techniques
- SDS-PAGE, one dimension (PAGE) and two dimension electrophoresis
- Isoelectric focusing
- Gel filtration chromatography, affinity chromatography
- High voltage electrophoresis, agarose electrophoresis, capillary electrophoresis
(D) Isotope Tracer Technique
- Basic concepts and principles
- Advantages, limitations, and applications in biochemistry
(E) Enzymes
- Enzyme classification, isoenzymes
- DNA polymerase
- Enzyme kinetics (basic)
(F) Blood and Other Body Fluid and Hair Examination (Human and Animal)
- Blood group system
- Semen examination, saliva, urine, hair, sweat
- Biological stains and their preservation
Important Topics for MPSC Assistant Chemical Analyser Exam 2026
| Analytical Chemistry | Sampling, concentration calculations, calibration, validation, statistical treatment, titrimetry, gravimetric analysis and separation techniques. |
|---|---|
| Inorganic Chemistry Techniques | AAS, AES, ICP-AES, ICP-MS, UV-Visible spectroscopy, ion exchange chromatography, SEM-EDS and electroanalytical techniques. |
| Organic Chemistry Techniques | UV-Visible, FTIR, Raman, NMR and mass spectrometry along with chromatography and forensic organic analysis. |
| Biochemistry Techniques | Biomolecules, molecular biology, electrophoresis, chromatography, isotope tracer techniques, enzymes and examination of blood, body fluids and hair. |
How to Prepare for MPSC Assistant Chemical Analyser Exam 2026
- Start with the four core units and prepare a subject-wise study timetable covering every topic mentioned in the syllabus.
- Focus on instrumental techniques, their principles and applications, including ICP-MS, GC-MS, HPLC and SEM-EDS.
- Revise statistical treatment of results separately, especially t-test, F-test, standard deviation and confidence interval, as these topics require calculation-based practice.
- Prepare short notes on important separation and electrophoresis techniques used in biochemistry.
- Practice previous year question papers of similar forensic and analytical chemistry examinations, wherever available, to understand the expected difficulty level and question pattern.
- Revise important spectroscopy, chromatography and forensic analysis techniques regularly to improve retention.
- Keep checking the official MPSC website for updates regarding the detailed exam pattern, marks distribution and examination date.
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